| 兔血清诱导Shope肿瘤细胞凋亡的实验研究 |
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| 引用本文： | 邵世和,李咏梅,陈曦,程文增,张成义,刘雅琴.兔血清诱导Shope肿瘤细胞凋亡的实验研究[J].北华大学学报(自然科学版),1998(1). |
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| 作者姓名： | 邵世和 李咏梅 陈曦 程文增 张成义 刘雅琴 |
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| 作者单位： | 吉林医学院微生物免疫教研室!吉林132001(邵世和，李咏梅，陈曦)，吉林医学院药理教研室(程文增，张成义)，吉林医学院免疫教研室(刘雅琴) |
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| 基金项目： | 吉林省教委计划资助项目 |
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| 摘 要： | 目的：前期实验结果已证明兔血清对Ｓｈｏｐｅ肿瘤细胞具有伤作用。本实验拟从细胞凋亡角度探讨兔血清杀伤该细胞的机理。方法：通过电子显微镜、激光共聚焦显微镜、琼脂糖凝胶电泳及流式细胞仪检测等方法，观察了兔血清诱导细胞凋亡的可能性。结果：经１０％兔血清处理３ｈ的中等分化程度的Ｓｈｏｐｅ肿瘤细胞（ＳＣＢ－６ａ）在电镜、激光共聚焦显微镜下均可见典型的细胞凋亡的形态学特征；１．５％琼脂糖凝胶电泳呈现明显的“阶梯状”图谱（ＤＮＡ ｌｅｄｄｅｒ）；ＰＩ染色后经流式细胞仪检测显示大量亚二倍体细胞（凋亡细胞）。结论：兔血清诱导Ｓｈｏｐｅ肿瘤细胞凋亡可能是其伤细胞的作用机理之一。
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| 关 键 词： | 细胞凋亡 兔血清 shope肿瘤细胞 |
The experimental study on rabbit sera induced apoptosis in Shope carcinoma cells |
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| Shao Shihe,Li Yongmei,Chen Xi,Cheng Wenzeng,Zhang Chengyi.The experimental study on rabbit sera induced apoptosis in Shope carcinoma cells[J].Journal of Beihua University(Natural Science),1998(1). |
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| Authors: | Shao Shihe Li Yongmei Chen Xi Cheng Wenzeng Zhang Chengyi |
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| Abstract: | Objective: It was proved that there is a killing effect on the Shope carcinoma cells with rabbit sera in the past experimental results. This experiment is to study the mechanisms. Methods:The detection of electron and conforcus microscopc, agarose gel electrophoresis and flow cytometer were used in the experi- ment. Results: After 3 hours treated with 10% rabbit sera, the middle-differential Shop tumor cells (SCB-6a) showed typical apoptosis under conforcus and electron microscope; typical DNA ladder was shown with DNA agarose gel electrophoresis: lots of the sub-biploid cells (apoptotic cells) was shown by flow ey- tometer with PI cells with rabbit sera is to induce the apoptosis of the tumor cells. |
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| Keywords: | apoptosis rabbit sera Shope carcinoma cell |
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