| 一种新型分光光度计液槽的设计及其在临床生化检验中的应用 |
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| 引用本文： | 黄萍,邓雅斌,王华,李东辉.一种新型分光光度计液槽的设计及其在临床生化检验中的应用[J].分析科学学报,2009,25(3). |
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| 作者姓名： | 黄萍 邓雅斌 王华 李东辉 |
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| 作者单位： | 1. 厦门大学医学院抗癌研究中心,福建厦门,361005
2. 厦门大学医院,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金，福建省科技项目，现代分析科学教育部重点实验室资助项目 |
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| 摘 要： | 设计并制作了可容纳塑料离心管的新型分光光度计液池。液池适用的样品体积变化范围宽（10-500μL），尤其适合于生物临床样品、强碱性介质以及大批样品的分析。该装置应用于血清蛋白质（总蛋白、白蛋白）、尿素氮、葡萄糖的测定及聚合酶链式反应（PCR）反应管的均一性检验，取得了满意的结果。
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| 关 键 词： | 液槽 分光光度法 分光光度计 |
A New-designed Cuvette for Spectrophotometry and Its Applications in Clinical Assay |
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| HUANG Ping,DENG Ya-bin,WANG Hua,LI Dong-hui.A New-designed Cuvette for Spectrophotometry and Its Applications in Clinical Assay[J].Journal of Analytical Science,2009,25(3). |
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| Authors: | HUANG Ping DENG Ya-bin WANG Hua LI Dong-hui |
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| Institution: | 1.Cancer Research Center;Medical College;Xiamen University;Xiamen 361005;2.Xiamen University Hospital;Xiamen 361005 |
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| Abstract: | A new cuvette which is able to contain a plastic centrifuge tube or a PCR tube for directly spectrophotometric detection was designed and tested.Absorption characteristics of a plastic centrifuge tube in the cuvette was investigated.The tubes showed obvious absorption in ultra-violet region at wavelength less than 300 nm,indicating that the detection wavelength should be set above 400 nm when applying the cuvette for the assay of samples.Plastic tube in this new-designed cuvette showed excellent linear resp... |
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| Keywords: | Cuvette Spectroscopy Spectrophotometer |
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