Application of Supermacroporous Monolithic Hydrophobic Cryogel in Capturing of Albumin |
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| Authors: | Nesibe Avcibaşi Murat Uygun M Emin Çorman Sinan Akgöl Adil Denizli |
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| Institution: | (1) Ege Vocational and Training School, Ege University, Izmir, Turkey;(2) Department of Arts Chemistry, Faculty of Science, Adnan Menderes University, Aydin, Turkey;(3) Department of Biochemistry, Faculty of Science, Ege University, Izmir, Turkey;(4) Department of Chemistry, Faculty of Science, Hacettepe University, Ankara, Turkey; |
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| Abstract: | Supermacroporous poly{2-hydroxyethyl methacrylate-co-N,N-bis(2,6-diisopropylphenyl)-perylene-3,4,9,10-tetracarboxylic diimide]} poly(HEMA-co-DIPPER)] monolithic cryogel column was prepared by radical cryocopolymerization of HEMA with DIPPER as functional comonomer
and N,N′-methylene-bisacrylamide (MBAAm) as crosslinker directly in a plastic syringe for adsorption of albumin. The monolithic cryogel
contained a continuous polymeric matrix having interconnected pores of 10–50 μm size. Poly(HEMA-co-DIPPER) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium
swelling degree of the poly(HEMA-co-DIPPER) cryogel was 14.7 g H2O/g dry cryogel. Poly(HEMA-co-DIPPER) cryogel was used in the adsorption/desorption of albumin from aqueous solutions. The nonspecific adsorption of albumin
onto plain poly(HEMA) cryogel was very low (3.36 g/g polymer). The maximum amount of albumin adsorption from aqueous solution
in acetate buffer was 40.9 mg/g polymer at pH 5.0. It was observed that albumin could be repeatedly adsorbed and desorbed
with the poly(HEMA-co-DIPPER) cryogel without significant loss of adsorption capacity. |
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