Concurrent Detection of Protein Adsorption on Mixed Nanoparticles by Differential Centrifugal Sedimentation |
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| Authors: | Ruimin Wang Lan Chen Dexing Li Renxiao Liu Guanglu Ge |
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| Affiliation: | CAS Key Laboratory of Standardization and Measurement for Nanotechnology and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, China |
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| Abstract: | In mixtures of nanoparticles of various sizes or compositions, monitoring protein partitioning on their surfaces provides important information about particle–protein interactions during competitive adsorption. Utilizing the size‐resolving capability of differential centrifugal sedimentation, the adsorption of bovine serum albumin on multisize gold nanoparticles with diameters ranging from 20 to 100 nm or gold, silver, and silica nanoparticles with similar diameter can be concurrently observed. This method can be used to gain insight into nanoparticle–protein interactions based on analyses of curvature and relative abundance. |
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| Keywords: | bovine serum albumin shell competitive adsorption differential centrifugal sedimentation gold nanoparticles |
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