Metal–Polymer Hybrid Nanoparticles for Correlative High‐Resolution Light and Electron Microscopy |
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| Authors: | Martin Reifarth Elisabeth Preußger Ulrich S Schubert Rainer Heintzmann Stephanie Hoeppener |
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| Institution: | 1. Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Jena, Germany;2. Jena Center of Soft Matter (JCSM), Friedrich Schiller University Jena, Jena, Germany;3. Abbe Center of Photonics, Jena, Germany;4. Institute of Physical Chemistry, Friedrich Schiller University Jena, Jena, Germany;5. Leibniz Institute of Photonic Technology, Jena, Germany |
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| Abstract: | The combination of fluorescence microscopy and electron microscopy promises a deeper insight into the ultrastructural features of cell organelles, e.g., after drug administration. Both methods complement each other and provide, as a correlative approach, a keen insight into the fate of nanoparticles within the cell. Moreover, it represents a promising tool to determine alterations of the cellular environment as a response to particle uptake. However, the availability of suitable correlative markers is mandatory for such correlative approaches. In this contribution, the utilization of poly(ethylene imine) based metal–polymer hybrid particles labeled with small gold nanoparticles and Rhodamine B facilitating the observation of the particles by means of fluorescence as well as by transmission electron microscopy is suggested. Correlative light and electron microscopy is used to study uptake and intracellular fusion processes of endosomal/lysosomal structures. |
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| Keywords: | correlative light and electron microscopy gold nanoparticles poly(ethylene imine) scanning transmission electron microscopy structured illumination microscopy uptake studies |
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