| ALTERATION IN CHROMATIN CONFORMATION OF PROLIFERATING AND DIFFERENTIATING ENZYME GENES IN NORMAL MOUSE LIVER AND ASCITES HEPATOMA CELLS AND ITS RELATION TO THE GENE EXPRESSION OF ENZYMES |
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| 引用本文： | 李士谔,马恩陵,吴石君. ALTERATION IN CHROMATIN CONFORMATION OF PROLIFERATING AND DIFFERENTIATING ENZYME GENES IN NORMAL MOUSE LIVER AND ASCITES HEPATOMA CELLS AND ITS RELATION TO THE GENE EXPRESSION OF ENZYMES[J]. 中国科学B辑(英文版), 1988, 0(11) |
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| 作者姓名： | 李士谔 马恩陵 吴石君 |
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| 作者单位： | Faculty of Basic Medicine，Beijing Union Medical University，Institute of Basic Medical Sciences，Chinese Academy of Medical Sciences，Beijing，Faculty of Basic Medicine，Beijing Union Medical University，Institute of Basic Medical Sciences，Chinese Academy of Medical Sciences，Beijing，Faculty of Basic Medicine，Beijing Union Medical University，Institute of Basic Medical Sciences，Chinese Academy of Medical Sciences，Beijing |
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| 基金项目： | This work was supported by the State Science and Technology Committee for Basic Research. The enzyme activities were determined by Li Yinxiong and Yu Yue. |
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| 摘 要： | Nuclei from the normal mouse liver were partially digested with micrococcal nuclease, followed by DNA extraction, agarose gel clectrophoresis and dot blot hybridization with ~(32)P-labeled cDNA probes of CPS_1 and ACT complex, It was clearly shown that the CPS_1 genes were distributed on the monomer, dimer. and trimer of nucleosomes, while the genes coding for ACT complex were distributed on the condensed oligonucleosomes. An opposite manner of distribution of CPS_1 and ACT complex genes was, however, noted in the case of ascites hepatoma cells, in which the specific activity of ACT was 13 times higher than that in the normal liver, while that of CPS_1 was remarkably reduced. Similar patterns of change in mRNA level of CPS_1 and ACT complex were observed in the normal mouse liver and ascites hepatoma cells, indicating a close relationship between chromatin structure and gene expression of these enzymes.
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ALTERATION IN CHROMATIN CONFORMATION OF PROLIFERATING AND DIFFERENTIATING ENZYME GENES IN NORMAL MOUSE LIVER AND ASCITES HEPATOMA CELLS AND ITS RELATION TO THE GENE EXPRESSION OF ENZYMES |
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| LI SHI''''E MA ENLING AND Wu SHIJUN. ALTERATION IN CHROMATIN CONFORMATION OF PROLIFERATING AND DIFFERENTIATING ENZYME GENES IN NORMAL MOUSE LIVER AND ASCITES HEPATOMA CELLS AND ITS RELATION TO THE GENE EXPRESSION OF ENZYMES[J]. Science in China(Chemistry), 1988, 0(11) |
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| Authors: | LI SHI''''E MA ENLING AND Wu SHIJUN |
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| Abstract: | Nuclei from the normal mouse liver were partially digested with micrococcal nuclease, followed by DNA extraction, agarose gel clectrophoresis and dot blot hybridization with ~(32)P-labeled cDNA probes of CPS_1 and ACT complex, It was clearly shown that the CPS_1 genes were distributed on the monomer, dimer. and trimer of nucleosomes, while the genes coding for ACT complex were distributed on the condensed oligonucleosomes. An opposite manner of distribution of CPS_1 and ACT complex genes was, however, noted in the case of ascites hepatoma cells, in which the specific activity of ACT was 13 times higher than that in the normal liver, while that of CPS_1 was remarkably reduced. Similar patterns of change in mRNA level of CPS_1 and ACT complex were observed in the normal mouse liver and ascites hepatoma cells, indicating a close relationship between chromatin structure and gene expression of these enzymes. |
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| Keywords: | gene expression chromatin conformation CPS_1 ACT hepatoma. |
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