Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111) |
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Authors: | Böcking Till Kilian Kristopher A Gaus Katharina Gooding J Justin |
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Institution: | School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia. |
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Abstract: | Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si-H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA. |
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