纳米比色法测定左氧氟沙星含量的仪器分析实验设计

利用左氧氟沙星诱导十二烷基硫酸钠(SDS)稳定的银纳米粒子(SDS-AgNPs)聚集变色现象建立了灵敏的测定左氧氟沙星含量的新方法。当左氧氟沙星加入到SDS-AgNPs溶液中时,SDS-AgNPs发生团聚导致其吸收峰发生红移并且吸收强度下降,溶液颜色也由黄色变为红色,利用溶液颜色变化可进行裸眼以及紫外-可见分光光度法检测左氧氟沙星。该实验绿色环保、简单易行,操作方便,成本低,有利于培养学生创新和进行科学研究的能力,适用于本科生实验教学。

Experimental Design:Colorimetric Detection of Levofloxacin Based on Silver Nanoparticles

A sensitive method based on the sodium dodecyl sulfate- stabilized silver nanoparticles (SDS-AgNPs) for detection of levofloxacin was reported. Levofloxacin could rapidly induce the aggregation of SDS-AgNPs, thereby resulting in a yellow-to-red color change. The color change could be monitored by naked eye and UV- Vis spectrophotometry. The presented method is convenient,simple, cost- effective and suitable for comprehensive laboratory teaching to develop students' innovation and scientific research ability.