Fluorous solvent for cell culture |
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Authors: | Maria Carmelita Z Kasuya Xiaonan Wen Kenichi Hatanaka Kageyasu Akashi |
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Institution: | aInstitute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan;bThe Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan |
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Abstract: | Incubation of mouse melanoma B16 cells in fluorous solvents with low boiling point such as perfluoromethylcyclohexane, 1,1,1,3,3,3-hexafluoro-2-propanol, ethylpentafluoropropionate resulted in cell death. However, cells lived up to 2 days in fluorous alcohols such as 2,2,3,3,4,4,5,5-octafluoro-1-pentanol and 3,3,4,4,5,5,6,6,6-nonafluoro-1-hexanol with relatively higher fluorine content. Remarkably, cells survived deprived of nutrition up to 4 days when incubated in 2,2,3,3,4,4,5,5,6,6,6-undecafluoro-1-hexanol or in 2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoroheptanol that have the most number of fluorine atoms (11 and 12, respectively) among the perfluoroalkyl alcohols used, and with boiling points of 128 °C and 169 °C, respectively. |
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Keywords: | Perfluorocarbons Fluorous solvents Melanoma B16 cells Cytotoxicity |
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