Single-step purification of different lipases from Staphylococcus warneri |
| |
Authors: | Giandra Volpato Marco Filice Marco AZ Ayub Jose M Guisan Jose M Palomo |
| |
Institution: | 1. Departamento de Biocatalisis, Instituto de Catalisis (CSIC), Madrid, Spain;2. Food Science and Technology Institute, Federal University of Rio Grande do Sul State, Porto Alegre, RS, Brazil |
| |
Abstract: | Three different lipases from the extract crude of Staphylococcus warneri have been purified by specific lipase–lipase interactions using different lipases (TLL, RML, PFL, BTL2) covalently attached to a solid support as adsorption matrix. BTL2 immobilized on glyoxyl-DTT adsorbed selectivity only a 30 kDa lipase from the crude, which was desorbed by adding 0.1% triton X-100. Using glyoxyl-PFL as matrix, two new lipases (28 and 40 kDa) were adsorbed, and completely pure 40 kDa lipase was obtained after desorption using 0.01% triton, whereas 28 kDa lipase was desorbed after the incubation of the lipase matrix with 3% detergent. When using other matrixes as glyoxyl-TLL or glyoxyl-RML, different lipases were adsorbed. This methodology could be a very efficient and useful method to purify several lipases from crude extracts from different sources. |
| |
Keywords: | Interfacial activation Purification Interaction lipase&ndash lipase Staphylococcus warneri |
本文献已被 ScienceDirect 等数据库收录! |
|