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Characterization and classification of matrix effects in biological samples analyses |
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| Authors: | Ivano Marchi Véronique Viette Flavia Badoud Marc Fathi Martial Saugy Serge Rudaz Jean-Luc Veuthey |
| Institution: | 1. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d’Yvoy 20, 1211 Geneva 4, Switzerland;2. Laboratory Medicine Service, University Hospitals of Geneva, rue Micheli-Du-Crest 24, 1211 Geneva 14, Switzerland;3. Swiss Laboratory for Doping Analyses, Institut Universitaire de Médecine Légale, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Chemin des Croisettes 22, 1066 Epalinges, Switzerland |
| Abstract: | An exhaustive classification of matrix effects occurring when a sample preparation is performed prior to liquid-chromatography coupled to mass spectrometry (LC–MS) analyses was proposed. A total of eight different situations were identified allowing the recognition of the matrix effect typology via the calculation of four recovery values. A set of 198 compounds was used to evaluate matrix effects after solid phase extraction (SPE) from plasma or urine samples prior to LC–ESI-MS analysis. Matrix effect identification was achieved for all compounds and classified through an organization chart. Only 17% of the tested compounds did not present significant matrix effects. |
| Keywords: | Matrix effects Ion suppression Plasma Urine Sample preparation SPE MS |
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