Murine fecal proteomics: A model system for the detection of potential biomarkers for colorectal cancer |
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| Authors: | Ching-Seng Ang Julie Rothacker Heather Patsiouras Antony W Burgess Edouard C Nice |
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| Institution: | 1. Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, PO Box 2008, Royal Melbourne Hospital, Australia;2. The Joint Proteomics Facilities, Ludwig Institute for Cancer Research and The Walter and Eliza Hall Institute of Medical Research, Australia;3. The University of Melbourne, Australia |
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| Abstract: | Tumor related products shed into the feces offer a potential source of biomarkers for the detection of colorectal cancer (CRC). Using SDS-PAGE followed by nanoflow reversed-phased LC–MS/MS to analyse fecal samples from ApcMin/+ mice (that develop spontaneous multiple intestinal neoplasia with age) we have identified 336 proteins (115 proteins of murine origin, 201 from fecal bacteria, 18 associated with food intake and 2 of apparent parasitic origin). 75% of the murine proteins identified in this study are predicted to be extracellular or associated with the cell plasma membrane. Of these proteins, a number of the murine homologues of colorectal cancer associated proteins (CCAP) such as hemoglobin, haptoglobin, hemopexin, alpha-2-macroglobulin and cadherin-17 have been identified, demonstrating the potential of fecal proteomics for detecting potential biomarkers and paving the way for subsequent MS/MS based biomarker studies on similar human samples. |
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| Keywords: | Murine fecal proteomics ApcMin/+ mice Colorectal cancer Biomarkers Nano-LC&ndash ESI-MS/MS analysis |
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