不同功能化基团修饰的硅壳纳米颗粒分散性研究

采用反相微乳液体系中功能化基团同步修饰(油相修饰)以及反相微乳液制备纳米颗粒后再通过功能化基团后续修饰(水相修饰)的方法分别制备了纯硅壳纳米颗粒(SiNP)、氨基化硅壳纳米颗粒(NSiNP)、羧基化硅壳纳米颗粒(CSiNP)和聚乙二醇硅壳纳米颗粒(PSiNP). 通过沉降时间和离心速度观察了不同方法获得的不同功能化基团修饰的硅壳纳米颗粒在水中的分散及稳定性, 并采用激光粒度仪、透射电子显微镜对分散效果进行了分析. 结果表明, 采用同一修饰方法分别制备的纳米颗粒在水中的分散及稳定性顺序是CSiNP≥PSiNP>SiNP>NSiNP; 油相修饰法获得的CSiNP和PSiNP的分散性要优于水相修饰法获得的. PSiNP和CSiNP在Hela细胞表面的非特异性吸附非常小, 而NSiNP却显示了强烈的细胞非特异性吸附.

Studies on Dispersibility of Different Functionalized Silica Nanoparticles

Silica nanoparticles(SiNP), amino-terminated silica nanoparticles(NSiNP), carboxyl-terminated silica nanoparticles(CSiNP) and PEG-terminated nanoparticles(PSiNP) were prepared via the synchronous modification of functional group in water-in-oil microemulsion(hereinafter referred to being modified in oil) and the modification of functional group on the well-prepared SiNP(hereinafter referred to being modified in water) respectively. The dispersibility of different functionalized silica nanoparticles in water suspension was evaluated with sediment velocity and centrifugation velocity. And further analysis of the dispersion behavior was demonstrated through the TEM imaging and hydrodynamic size analyses. The results indicat that the following order of dispersion was observed: CSiNP≥PSiNP>SiNP>NSiNP, when the silica nanoparticles were modified with the same method. In addition, for CSiNP and PSiNP, the dispersibility of the two nanoparticles modified in oil was superior to that of modified in water. At the same time, the nonspecific adsorption of the different functionalized silica nanoparticles modified in oil with the Hela cells was also investigated. There was almost no nonspecification with Hela cells to CSiNP and PSiNP. However, the NSiNP were adsorbed to the Hela cells extensively. These results played important roles in the preparation and further modification of silica nanoparticles that would be well suited in biomedicine.