Analysis of wine phenolics by high-performance liquid chromatography using a monolithic type column |
| |
| Authors: | Castellari Massimo Sartini Elisa Fabiani Alessandra Arfelli Giuseppe Amati Aureliano |
| |
| Institution: | Department of Biochemistry, Biomedical Center, Uppsala University, Box 576, SE-751 23 Uppsala, Sweden |
| |
| Abstract: | A polyacrylamide gel crosslinked with allyl-β-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-β-cyclodextrin can easily be synthesized in one step from allylglycidylether and β-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m−1. The resolution was almost independent of the concentration of the crosslinker (allyl-β-cyclodextrin) — in sharp contrast to gels crosslinked with N,N′-methylenebisacrylamide. |
| |
| Keywords: | DNA Polyacrylamide Allyl-β-cyclodextrin |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
