随机扩增多态性DNA技术在生命科学炽的应用

DNA分子标记是DNA水平上遗传变异的直接反映，随机扩增多态性DNA（RAPD）技术，是新发展起来的一种DNA分子标记方法。文中详细阐述了RAPD技术的原理，进一步与限制性片段长度多态性（Restriction FragmentLength Polmorphism,RFLP)技术相比，得出它具有快速，简便和对材料要求不高等特点，最后讨论了RAPD技术在生命科学研究中各个方面的广泛应用，包括种基因组的分子谱图建、系统进化发育以及基因定位研究等。

RAPD Molecular Markers and Its Applications in Life Science

A DNA polymorphison assay, random amplified polymorphic DNA (RAPD), was developed in 1990 based on amplification by the polymerase chain reaction (PCR) of random DNA segments and single primers of arbitrary uncleotide sequnce. The major advantage of this assay, contrasting with the restriction fragment length polymorphism (RFLP), is that there is no requirement for any specific sequence information on the target genome, as well as rapidity and simplicity.It has been widespread applied in life science researches such as the creation of high density genetic mapping, molecular phylogenetic study and gene location.