Direct electrochemistry and electrocatalysis of hemoglobin on a gold ion implantation-modified indium tin oxide electrode |
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Authors: | Mingzhe Jia Fenfen Liang Jiao Jiao Shuoqi Li Jingbo Hu |
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Affiliation: | 2. College of Chemistry, Beijing Normal University, Beijing, 100875, People’s Republic of China 1. Key Laboratory of Beam Technology and Material Modification of Ministry of Education, Beijing Normal University, Beijing, 100875, People’s Republic of China
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Abstract: | In this paper, a gold nanoparticle-modified indium tin oxide electrode (Au/ITO) was prepared without the use of any cross-linker or stabilizer reagent. The prepared Au/ITO was used as a new platform to achieve the direct electron transfer between Hb and the modified electrode. The proposed electrode exhibited a pair of well-defined redox peaks with a formal potential of ?0.073 V (vs. Ag/AgCl). The immobilized Hb showed excellent electrocatalytic activity toward H2O2 and the electrocatalytic current values were linear with the increasing concentration of H2O2 ranging from 1.0?×?10?6?M to 7.0?×?10?4?M. The detection limit was 2.0?×?10?7?M (S/N?=?3) and the Michaelis–Menten constant was calculated to be 0.2 mM. The proposed electrode also showed high selectivity, long-term stability, and good reproducibility. |
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