A blue fluorescent antibody-cofactor sensor for mercury |
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| Authors: | Matsushita Masayuki Meijler Michael M Wirsching Peter Lerner Richard A Janda Kim D |
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| Institution: | Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA. kdjanda@scripps.edu |
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| Abstract: | reaction: see text] A chemically programmed antibody sensor, consisting of a stilbenyl boronic acid cofactor and monoclonal antibody EP2-19G2, provides a new method of mercury detection. The fluorescent antibody sensor generates an intense powder blue fluorescence when bound to the stilbenyl boronic acid cofactor; however, it is quenched in the presence of Hg(2+) ions. The EP2-19G2-cofactor biosensor provides micromolar sensitivity and selectivity toward Hg(2+) ions over a wide range of metal ions in aqueous solution. |
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