Using mass spectrometry to detect buffalo salivary odorant-binding protein and its post-translational modifications |
| |
Authors: | Rajkumar R Karthikeyan K Archunan G Huang P H Chen Y W Ng W V Liao C C |
| |
Institution: | Center for Pheromone Technology, Department of Animal Science, Bharathidasan University, Trichirappalli 620 024, Tamilnadu, India. |
| |
Abstract: | A large number of mammalian odorant-binding proteins, which are lipocalins, have been studied. These proteins participate in peri-receptor events by selecting and carrying odorant molecules. The present study aimed at identifying the buffalo salivary odorant-binding protein (sOBP), and to determine its post-translational modification using mass spectrometry. The buffalo salivary 21 kDa protein was initially separated adopting sodium dodecyl sulfate-polyacrylamide gel electrophoresis and it was identified as sOBP with high statistical reliability using liquid chromatography/tandem mass spectrometry (LC/MS/MS) and SEQUEST, for the first time. Further, the post-translationally modified peptides were screened adopting MS/MS. A total of four post-translational modifications, namely glycation at lysine-(59), hydroxylation at lysine-(134), ubiquitination at lysine-(121), and dihydroxylation in lysine-(108), were recorded. Moreover, these modifications have not been identified in buffalo salivary odorant-binding protein. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|