DNA molecular configurations in flows near adsorbing and nonadsorbing surfaces |
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Authors: | Lei?Li Hua?Hu Email author" target="_blank">Ronald?G?LarsonEmail author |
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Institution: | (1) Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA;(2) Present address: Dept of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI 48109, USA |
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Abstract: | We investigate experimentally -phage and T2-coliphage DNA molecules near both non-adsorbing glass and adsorbing 3-aminopropyltriethoxysilane (APTES)-coated glass surfaces in a simple steady shearing flow generated by a torsional flow cell. The DNA molecular deformations near the surface are found to be considerably weaker than in bulk flow at the same shear rate. This affects the DNA molecule s deposition and stretching on the adsorbing surface. Surprisingly, for a simple shearing flow in the torsional shearing device, the observed stretch, for molecules both near (<10 µm) the surface and adsorbed to it, is much less than predicted by simulations. |
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Keywords: | Shearing flow DNA molecule Glass surface Bulk and surface behavior Molecular deformation |
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