Nonaqueous capillary electrophoresis using a titania-coated capillary |
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Authors: | Xu Li Feng Yu-Qi Shi Zhi-Guo Da Shi-Lu Ren Yao-Yao |
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Affiliation: | Department of Chemistry, Wuhan University, Wuhan 430072, China. |
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Abstract: | In this work, an ordered mesoporous titania film was introduced to coat a capillary by means of the sol-gel technique. Its electroosmotic flow (EOF) property was investigated in a variety of nonaqueous media (methanol, formamide and N,N'-dimethylformamide and mixtures of methanol and acetonitrile). The titania-coated capillary exhibited a distinctive EOF behavior, the direction and magnitude of which were strongly dependent on various parameters such as the solvent composition, apparent pH (pH*) and the electrolytes. The nonaqueous capillary electrophoresis separation of several alkaloids was investigated in the positively charged titania-coated capillary. Comparison of separation between coated and uncoated capillaries under optimal nonaqueous conditions was also carried out. |
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Keywords: | Nonaqueous capillary electrophoresis Background electrolyte composition Coated columns Titania Alkaloids |
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