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| 一种pH敏感相分离高分子在免疫分析中的应用 | |
| 引用本文： | 柏正武,卓仁禧,周宜开.一种pH敏感相分离高分子在免疫分析中的应用[J].离子交换与吸附,2004,20(2):165-170. |
| 作者姓名： | 柏正武 卓仁禧 周宜开 |
| 作者单位： | 1. 武汉化工学院化工与制药系,武汉,430073;武汉大学化学系,武汉,430072 2. 武汉大学化学系,武汉,430072 3. 华中科技大学同济医学院公共卫生学院,武汉,430030 |
| 摘 要： | 将乙肝表面抗体与N-羟基琥珀酰亚胺丙烯酸酯反应，生成丙烯酰基抗体．丙烯酰基抗体与甲基丙烯酸、丙烯酰胺共聚，生成pH敏感的三元共聚物．三元共聚物溶解．沉淀的临界pH值为3．8．三元共聚物上的抗体能与表面抗原、酶标抗体形成夹心复合物．利用三元共聚物可逆的溶解．沉淀特性，能实现在均相条件下，进行免疫反应，在异相条件下，进行免疫复合物的分离． |
| 关 键 词： | pH敏感相分离高分子 免疫分析 环境敏感高分子 乙肝表面抗原 聚【甲基丙烯酸-丙烯酰胺-（N-羟基琥珀酰亚胺丙烯酸酯）】 三元共聚物 |
| 文章编号： | 1001-5493(2004)02-0165-06 |
| 修稿时间： | 2003年10月8日 |
THE APPLICATION OF POLY[METHACRYLIC ACID-CO-ACRYLAMIDE-CO-(N-ACRYLOXYSUCCINIMIDE)] FOR IMMUNOASSAY |
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| BAI Zhengwu, ZHUO Renxi ZHOU Yikai.THE APPLICATION OF POLY[METHACRYLIC ACID-CO-ACRYLAMIDE-CO-(N-ACRYLOXYSUCCINIMIDE)] FOR IMMUNOASSAY[J].Ion Exchange and Adsorption,2004,20(2):165-170. | |
| Authors: | BAI Zhengwu ZHUO Renxi ZHOU Yikai |
| Affiliation: | BAI Zhengwu1,2 ZHUO Renxi2 ZHOU Yikai3 1 Department of Chemical & Pharmaceutical Engineering,Wuhan Institute of Chemical Technology,Wuhan 430073,China 2 Department of Chemistry,Wuhan University,Wuhan,430072,China 3 School of Public Heath,Tongji Medical College,Hua Zhong University of Science & Technology,Wuhan 430030,China |
| Abstract: | A pH-sensitive terpolymer was prepared by copolymerization of methacrylic acid, acrylamide and acryloyl-HBsAb that was obtained through the reaction between N-acryloxysuccinimide and HBsAb. The critic pH value for phase change of this polymer was determined. The polymer could dissolve in a solution when pH value was above 3.8, and could precipitate almost completely when pH value was below 3.4. A sandwich immuno-complex was formed by the interaction of HBsAb, HBsAg and HRP-labeled HBsAb. Precipitation for separation and dissolution for immunoassay were successfully effected by the change of pH value of a solution. |
| Keywords: | pH-Sensitive polymer Terpolymer Reversible dissolution-precipitation Immunoassay |
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