Artemisinin evaluation in Romanian Artemisia annua wild plants using a new HPLC/MS method |
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Authors: | Ivanescu B Vlase L Corciova A Lazar M I |
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Institution: | Department of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy Gr. T. Popa, 16 Universitatii Street, 700150, Iasi, Romania. biancaivanescu@yahoo.com |
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Abstract: | Artemisinin, a sesquiterpene lactone from Artemisia annua L., has received considerable attention in the last few decades as a potent antimalarial drug. Artemisinin has rather low toxicity; it is effective against drug-resistant Plasmodium species and against cerebral malaria. This study reports the development of a rapid and sensitive assay for the quantification of artemisinin in A. annua by reversed phase HPLC/MS. In the selected optimal experimental conditions, artemisinin exhibited a well-defined chromatographic peak with a retention time of 2 ± 0.2 min. The chromatographic signal shows a linear dependence with artemisinin concentration, enabling the use of this signal for artemisinin quantification according to the following regression equation: y = 2665.40x - 14697.61. The correlation coefficient (R(2)) was 0.9989. For every concentration within the range of the standard curve (0.1-2 μg mL(-1)), accuracy was between 95 and 104%. Artemisinin content in Romanian A. annua wild plants varies between 0.17 and 0.21% (dry weight basis). |
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