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Cyclodextrins as hosts for poorly water-soluble compounds in enzyme catalysis |
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| Authors: | Roque Bru Jose M López-Nicolás Estrella Núñez-Delicado Dolores Nortes-Ruipérez Alvaro Sánchez-Ferrer Francisco Garciá-Carmona |
| Institution: | (1) Departamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia, Campus de Espinardo, E-30001 Murcia, Spain |
| Abstract: | The capability of cyclodextrins to enhance greatly the solubility in water of poorly water-soluble substances makes them an ideal alter native for investigating the expression of enzyme activity with such substrates in aqueous solution. This capability is demonstrated by using soybean lipoxygenase with linoleic acid/β-cyclodextrin and di ethylstilbestrol/γ-cyclodextrin, and cholesterol oxidase with choles terol/methyl-β-cyclodextrin. |
| Keywords: | Cyclodextrins linoleic acid diethylstilbestrol cholesterol lipoxygenase cholesterol oxidase |
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