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A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue |
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| Authors: | Xiaohu Xia Ye Xu Rongqin Ke Heng Zhang Mingqiang Zou Wei Yang Qingge Li |
| Affiliation: | 1. Engineering Research Centre of Molecular Diagnostics, Ministry of Education; Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen, Fujian, 361005, China 2. The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Georgia Institute of Technology, Atlanta, GA, 30332, USA 3. Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518067, China 4. Chinese Academy of Inspection and Quarantine, Beijing, 100123, China |
| Abstract: | A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is ~100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples. A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol (CAP) residue was developed, of which limit of detections (LODs) can reach low levels of 0.25 and 0.03 ng/mL, respectively, in the qualitative and quantitative modes. |
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