| Preliminary Fluorimetric Screening of Fourteen Palladium Complexes as Potential Antitumor Agents |
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| 作者姓名： | 林辉祥 李志良 戴桂林 毕琼斯 俞汝勤 |
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| 作者单位： | Department of Chemistry and Chemical Engineering，Hunan University，Changsha 410082，PRC，Department of Chemistry and Chemical Engineering，Hunan University，Changsha 410082，PRC，Department of Chemistry and Chemical Engineering，Hunan University，Changsha 410082，PRC，Department of Chemistry and Chemical Engineering，Hunan University，Changsha 410082，PRC，Department of Chemistry and Chemical Engineering，Hunan University，Changsha 410082，PRC |
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| 基金项目： | Project supported by the National Education Commission Foundation and the National Natural Science Foundation of China. |
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| 摘 要： | Making use of the fact that the combination of a drug substance with DNA may inhibit the duplication, synthesis and proliferation of DNA and the consistency of the in vivo and in vitro interactions, the authors worked out a preliminary screening method for testing complex agents as potential antitumor drugs using ethidium bromide as a fluorescence probe. In this report, the method was applied for in vitro testing fourteen synthesized palladium(Ⅱ)/phenanthroline/amino acid/chloride complexes as potential non-platinum antitumor agents. The fluorimetric screening method was compared with methylene blue tube test and trypan blue dye exclusion assay. All three methods gave agreeable results. Among the complexes tested, Pd(phen)(lys)]Cl, Pd(phen)(arg)]Cl and Pd(phen)(pro)]Cl showed antineoplastic ratios for animal tumor S-180 56%, 50% and 48%, respectively, in accordance with the order of their binding constants with DNA, 7.96×10~6, 4.52×10~6 and 1.0×10~6, respectively. The test results show that fluor
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| 关 键 词： | fluorimetric screening palladium(Ⅱ) complexes preliminary screening of antitumor drugs fluorescence probe. |
Preliminary Fluorimetric Screening of Fourteen Palladium Complexes as Potential Antitumor Agents |
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| LIN Hui-Xiang LI Zhi-Liang DAI Gui-Lin BI Qiong-Si and YU Ru-Qin.Preliminary Fluorimetric Screening of Fourteen Palladium Complexes as Potential Antitumor Agents[J].Science in China(Chemistry),1993(10). |
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| Authors: | LIN Hui-Xiang LI Zhi-Liang DAI Gui-Lin BI Qiong-Si and YU Ru-Qin |
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