| 非极性流动相高效液相色谱法定量测定辅酶Q10 |
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| 引用本文： | 孙心齐,焦贺贤,王金中,程岚霞,余海侠. 非极性流动相高效液相色谱法定量测定辅酶Q10[J]. 化学研究, 2004, 15(3): 56-58 |
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| 作者姓名： | 孙心齐 焦贺贤 王金中 程岚霞 余海侠 |
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| 作者单位： | 河南大学,化学化工学院,河南,开封,475001;河南大学,化学化工学院,河南,开封,475001;河南大学,化学化工学院,河南,开封,475001;河南大学,化学化工学院,河南,开封,475001;河南大学,化学化工学院,河南,开封,475001 |
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| 基金项目： | 河南大学重点学科资助项目. |
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| 摘 要： | 采用高效液相色谱外标法定量测定辅酶Q10的含量.色谱条件:4.6mm×25cmZorbox CN柱;流动相:V(正己烷)∶V(异丙醇)=99.5∶0.5;流速:1.0mL·min-1;紫外检测器275nm下检测;ABS:0.04.样品检测线性范围为8μg～0.05μg;相关系数r=0.9998;回收率100.35%;精密度RSD=1.48%.
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| 关 键 词： | 非极性流动相 液相色谱 辅酶Q10 定量分析 |
| 文章编号： | 1008-1011(2004)03-0056-03 |
| 修稿时间： | 2004-03-11 |
Quantitative Determination of Ubiquinone-10 by High Performance Liquid Chromatography with Nonpolar Liquid Phase |
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| SUN Xin-qi,JIAO He-xian,WANG Jin-zhong,CHENG Lan-xia,YU Hai-xia. Quantitative Determination of Ubiquinone-10 by High Performance Liquid Chromatography with Nonpolar Liquid Phase[J]. Chemical Research, 2004, 15(3): 56-58 |
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| Authors: | SUN Xin-qi JIAO He-xian WANG Jin-zhong CHENG Lan-xia YU Hai-xia |
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| Abstract: | The content of ubiquinone-10 was determined by HPLC with nonpolar liquid phase. The column used was Zorbox-CN(4.6 mm×25 cm). The UV detector was set at 275 nm. The eluate was a mixture of n-hexane and isopropanol (V (n-hexane )/ V( isopropanol)=99.5:0.5) with a flow rate of 1.0 mL·min~(-1). The results showed that the linear range was 8μg～0.05μg,r=0.9998. The average recovery of ubiquinone-10 was 100.35%. The relative standard deviation of accuracy was (1.48%). |
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| Keywords: | nonpolar liquid phase high performance liquid chromatography ubiquinone-10 quantitative analysis. |
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