氨基酸植物组织传感器的研究

选择苹果及仙人球的组织切片作生物催化材料,同氨气敏电极组合,研制了2种对L-谷氨酰胺及L-天冬酰胺选择响应的新型的组织传感器。研究和讨论了传感器的最佳工作条件。用该组织传感器测定了L-天冬酰胺脱氨酶和L-谷氨酰胺脱氨酶的动力学参数K_m和V_m。

Biosensors Based on the Tissue of Plant as Catalytic Material for Amino Acid

Two new tissue electrodes are described in this paper. It is shown that a portion of intact tissue from apple or cactus can be coupled with potentiometric ammonia gas sensing electrode to prepare sensors for some amino acids with good response properties. Their optimum operational conditions and properties with respect to buffer sort, pH of the bulk solution, selectivity, linearity, stability, sensitivity and response time are studied and discussed. At the same time, the dynamic parameters, namely Km and Vm, of L-glutamine deaminase and L-asparagine deaminase have been obtained by means of these tissue electrodes.