| 代谢组学方法研究奶牛注射氯霉素后牛奶中的生物标志物 |
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| 引用本文： | 王世成,李国琛,王颜红,胡小燕,张红.代谢组学方法研究奶牛注射氯霉素后牛奶中的生物标志物[J].分析化学,2010,38(8). |
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| 作者姓名： | 王世成 李国琛 王颜红 胡小燕 张红 |
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| 作者单位： | 中国科学院沈阳应用生态研究所,沈阳,110016 |
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| 基金项目： | 中科院知识创新重要方向项目，沈阳市科技计划项目 |
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| 摘 要： | 建立了高效液相色谱-质谱(HPLC-MS)技术研究牛奶代谢组学的方法.样品用乙腈提取,经浓缩富集,采用HPLC-MS技术研究了注射氯霉素后牛奶中内源性代谢物随时间的变化情况.结果表明,注射氯霉素后,牛奶的代谢指纹图谱中8个谱峰的变化随给药及休药时间呈现一定升降规律;采用偏最小二乘判别分析法(PLS-DA)可将给药组与空白组样品完全分开;鉴定了对组间差异贡献较大的化合物为单羟基十八碳二烯酸(HODE),HODE可以作为注射氯霉素后奶牛体内代谢应激产生的内源性生物标志物.
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| 关 键 词： | 牛奶 氯霉素 代谢组学 生物标志物 单羟基十八碳二烯酸 |
Study of Potential Biomarkers in Milk from Cows Treated with Chloramphenicol by Metabonomic Approach |
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| WANG Shi-Cheng,WANG Yan-Hong,LI Guo-Chen,HU Xiao-Yan,ZHANG Hong.Study of Potential Biomarkers in Milk from Cows Treated with Chloramphenicol by Metabonomic Approach[J].Chinese Journal of Analytical Chemistry,2010,38(8). |
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| Authors: | WANG Shi-Cheng WANG Yan-Hong LI Guo-Chen HU Xiao-Yan ZHANG Hong |
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| Institution: | WANG Shi-Cheng,WANG Yan-Hong,LI Guo-Chen,HU Xiao-Yan,ZHANG Hong (Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016) |
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| Abstract: | HPLC-MS-based metabonomic approach was established for the metabonomic analysis of milk components between normal cows and cows treated with chloramphenicol. Endogenous metabolites of the milk samples were analyzed by HPLC-MS after the extraction and. The results showed that: (1) After chloramphenicol was injected,from the corresponding chromatographs,it was found that the treated group and the control group were significantly different,in which some peaks changed regularly with the medicine-treated time,(2... |
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| Keywords: | Milk Chloramphenicol Metabonomics Biomarker Hydroxyl-octadecadienoic acid |
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