Reactive cysteine is protonated in the triplet excited state of the LOV2 domain in Adiantum phytochrome3 |
| |
| Authors: | Sato Yoshiaki Iwata Tatsuya Tokutomi Satoru Kandori Hideki |
| |
| Institution: | Department of Materials Science and Engineering, Nagoya Institute of Technology, Showa-ku, Nagoya 466-8555, Japan. |
| |
| Abstract: | Phototropin is a plant blue-light sensor protein that possesses a flavin mononucleotide (FMN) as the chromophore in LOV domains. Its photoreaction is an adduct formation between FMN and a nearby cysteine that takes place in the triplet excited state of FMN. In this communication, we revealed that the reactive cysteine is protonated in the triplet excited state of the LOV2 domain of Adiantum phytochrome3 by means of low-temperature FTIR spectroscopy. Its hydrogen-bonding interaction is strengthened in the triplet excited state, presumably with the FMN chromophore. Such strong interaction drives adduct formation on a microsecond time scale. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
