基于银增强金标记物的阳极溶出伏安免疫分析用于补体第三成分的测定

分子印迹是制备对特定分子具有专一性结合能力的聚合物的技术，所制备的聚合物被称为分子印迹聚合物（Molecularly imprinted polymers，MIPs），此类聚合物在分离提纯、模拟酶和传感器等方面均显示出广阔的应用前景，迄今，小分子化合物的印迹技术已经十分成熟。

Surface Molecularly Imprinted Polymer Nanowires Used for Protein Recognition

Molecularly imprinted polymers(MIPs) are artificial, template-made receptors with the ability to recognize and to specially bind the target molecule. The advantage of stability of the polymer, ease of the preparation and low cost of these MIPs have led to their assessment as substitutes for antibodies or enzymes in chemical sensors, catalysis and separations. Although creating a MIPs against small molecules is straightforward now, imprinting of large structures, such as proteins and other biomacromolecules, is still a challenge. The major problem associated with the imprinting of such large structures lies on the restricted mobility of them within highly cross-linked polymer networks and the poor efficiency in rebinding. In this paper, we present a technique for the preparation of polymer nanowires with the protein molecule imprinted and binding sites on surface. These surface imprinting nanowires exhibit highly selective recognition for a variety of template proteins, including albumin, hemoglobin and cytochrome c. Since the protein imprinted sites are located on, or close to, the surface, these imprinted nanowires have a good site accessibility towards the target protein molecules. Furthermore, the large surface area of the nanowires results in larger protein molecules binding capacity of the imprinted nanowires compared to previously report surface imprinting MIPs.