| RETROVIRUS MEDIATED TRANSFER OF ANTISENSE HUMAN c-myc GENE INTO HUMAN ESOPHAGEAL CANCER CELLS SUPPRESSED CELL PROLIFERATION AND MALIGNANCY |
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| 作者姓名： | 叶昕 吴旻 |
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| 作者单位： | National Laboratory of Molecular Ontology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，National Laboratory of Molecular Ontology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC |
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| 基金项目： | Project supported by the State Science and Technology Commission of PRC |
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| 摘 要： | A retroviral vector, called pDAM3, containing the neomycin resistant gene and the antisense human c-myc gene fragment (the third exon and 3'flanking sequence) was constructed. pDAM3 was introduced into amphotropic packaging cells PA317 by the calcium phosphate precipitation method. Several G418-resistant PA317 clones were isolated. The virus titer of these cell lines was determined by infectivity of their cuhure fluid to NIH/3T3 cells. The highest titer obtained was 8×10~5 G418-resistant colony forming units/ml. Clonal and pooled G418-resistant PA317 colonies with high titers were expanded and analyzed by Southern blot for the presence of intact viral sequences. All cell lines were found to harbor the internal sequences of the pDAM3 vector without any rearrangement. Recombinant virus DAM3 infected human esophageal cancer cell line EC8712 efficiently. The DAM3-infected EC8712 (called EC-DAM3) was found to contain the full DAM3 sequence (4.8kb) by Southern blot analysis. Antisense myc RNA expressed in th
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RETROVIRUS MEDIATED TRANSFER OF ANTISENSE HUMAN c-myc GENE INTO HUMAN ESOPHAGEAL CANCER CELLS SUPPRESSED CELL PROLIFERATION AND MALIGNANCY |
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| YE XIN AND WU MIN.RETROVIRUS MEDIATED TRANSFER OF ANTISENSE HUMAN c-myc GENE INTO HUMAN ESOPHAGEAL CANCER CELLS SUPPRESSED CELL PROLIFERATION AND MALIGNANCY[J].Science in China(Chemistry),1992(1). |
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| Authors: | YE XIN AND WU MIN |
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| Abstract: | |
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| Keywords: | retroviral vector myc oncogene antisense RNA esophageal cancer cell |
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