Direct electrochemistry and electrocatalysis of hemoglobin immobilized by methacrylic acid |
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| Authors: | Junhui Xu Jia Zhao Fang Wang Qing Lu Yanxia Xu Shengshui Hu |
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| Affiliation: | (1) College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China;(2) State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, 100084, China |
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| Abstract: | The direct electrochemistry of hemoglobin (Hb) incorporated in methacrylic acid (MAA) film on a paraffin-impregnated graphite electrode (PIGE) was described. A pair of well-defined and quasi-reversible cyclic voltametric peaks are obtained. The formal potentials (E 0′) linearly depend on the pH of solution, indicating that the electron transfer was proton-coupled. Ultraviolet-visible (UV-Vis) spectra showed that the secondary structure of Hb in the MAA film was similar to individual Hb. The immobilized Hb retained its biological activity well and exhibited a nice response to the reduction of both NO2−, and H2O2, on the basis of which a new biosensor has been developed. Published in Russian in Elektrokhimiya, 2008, Vol. 44, No. 9, pp. 1079–1086. The text was submitted by the authors in English. |
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| Keywords: | hemoglobin methacrylic acid direct electrochemistry electrocatalysis biosensor |
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