Experimental setup for the determination of analytes contained in ultrasonically levitated drops |
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Authors: | O. Rohling C. Weitkamp B. Neidhart |
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Affiliation: | Institut für Physikalische und Chemische Analytik, GKSS-Forschungszentrum Geesthacht GmbH, Max-Planck-Stra?e 1, 21502 Geesthacht, Germany, DE
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Abstract: | An automated technique for the determination of analytes in an ultrasonically levitated sample of 2 to 5 μL volume has been developed. Contactless dosing of reagents or solvents into an ultrasonically levitated drop was realized via piezoelectric micropumps. Drop size was continually controlled with a programmed CCD camera. A diode array spectrometer designed for the use with optical fibers was used for absorption and fluorescence measurements. Determinations via direct absorption measurements following the method of standard addition and acid-base titrations with an absorption indicator and a fluorescent indicator were carried out. The mean consumption of sodium hydroxide added via a piezoelectric micropump in five successive titrations with 18 nmol of sulfuric acid inside a levitated drop (indicator bromothymol blue) had a relative standard deviation of 0.7 % and differed only by 0.2 % from the expected value. Received: 19 January 2000 / Revised: 28 February 2000 / Accepted: 3 March 2000 |
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