Surface‐enhanced Raman scattering spectroscopy for potential noninvasive nasopharyngeal cancer detection |
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| Authors: | Juqiang Lin Rong Chen Shangyuan Feng Jianji Pan Buhong Li Guannan Chen Shaojun Lin Chao Li Li‐qing Sun Zufang Huang Haishan Zeng |
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| Institution: | 1. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, 350007 China;2. Juqiang Lin, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China;3. E‐mail: jqlin@fjnu.edu.cn;4. Rong Chen, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China;5. E‐mail: chenr@fjnu.edu.cn;6. Fujian Provincial Tumor Hospital, Fuzhou, 350001 China;7. Fuzhou First Hospital, Fuzhou, 350014 China;8. Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada |
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| Abstract: | Combining membrane electrophoresis with surface‐enhanced Raman scattering (SERS) spectroscopy, the serum proteins were first purified and then mixed with silver nanoparticles to perform SERS spectral analysis. Therefore, the spectral signatures were enhanced to high‐fidelity SERS signatures because of the purification procedure of the first step. We used the method to analyze blood plasma samples from nasopharyngeal cancer patients (n = 43) and healthy volunteers (n = 33) for cancer detection. Principle component analysis of the SERS spectra revealed that the data points for the cancer group and the normal group form distinct, completely separated clusters with no overlap. Therefore, the nasopharyngeal cancer group can be unambiguously discriminated from the normal group, i.e., with both diagnostic sensitivity and specificity of 100%. These results are very promising for developing a label‐free, noninvasive, and reliable clinical tool for rapid cancer detection and screening. Copyright © 2011 John Wiley & Sons, Ltd. |
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| Keywords: | membrane electrophoresis surface‐enhanced Raman scattering (SERS) principal component analysis (PCA) blood plasma proteins nasopharyngeal cancer |
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