Electrochemical biosensor based on supported planar lipid bilayers for fast detection of pathogenic bacteria |
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| Institution: | 1. Computational Biology Lab, National Center for Bioinformatics, Quaid-i-Azam University Islamabad, Islamabad 45320, Pakistan;2. Structural and Computational Biology Research Group, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;3. Ph.D. Program in Bioinformatics and Computational Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;1. Chemistry Department, College of Applied Sciences, Umm Al-Qura University, Makkah, Saudi Arabia;2. Chemistry Department Faculty of Science, Mansoura University, Mansoura, Egypt;3. Chemistry Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, Saudi Arabia;4. Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, Saudi Arabia;5. Chemistry Department, Faculty of Applied Sciences, Taiz University, P.O. Box 82, Taiz, Yemen |
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| Abstract: | This paper presents a new ion-channel biosensor based on supported bilayer lipid membrane for direct and fast detection of Campylobacter species. The sensing element of a biosensor is composed of a stainless-steel working electrode, which is covered by artificial bilayer lipid membrane (BLM). Antibodies to bacteria embedded into the BLM are used as channel forming proteins. The biosensor has a strong signal amplification effect, which is defined as the total number of ions transported across the BLM. The total number of (univalent) ions flowing through the channels is 1010 ions s−1. The biosensor showed a very good sensitivity and selectivity to Campylobacter species. |
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