关于含酯基多孔聚合物小球固定相保留机制的探讨

通过比保留体积对数值与绝对温度倒数的直线关系,水和甲醇标准曲线的正截距、氘交换以及吸附系数和溶解系数的测定,证明至少对我们的聚合物固定相来说。表面吸附对保留值是决定性的因素。

Studies on the Retention Mechanism for Ester-Containing Porous Polymer Beads as Stationary Phase

The dependence of the logarithms of the specific retention volumes for C₅-C₈ alkanes and McReynolds standard compounds on the reciprocal temperature was investigated for two types of ester-containing polymer beads as stationary phase. Within the temperature ranges investigated the dependence is linear. Hence it could be assumed that the retention mechanism would not be with abrupt change.The calibration curves for water and methanol are linear, but the extrapolation to zero point yields a positive intercept which is attributed to the adsorption on the stationary phase.The hydrogen exchange between methanol -D₄ and D₂O on polymer beads was found by GC.Based on the relationship between the specific retention volume and specific surface area shown below V_g = K_iσ+ K₁ where K_i and K₁ represent the adsorption coefficient and partition coefficient, respectively, it was shown that adsorption on the surface of beads is the decisive factor determining the retention value, at least for our polymers.