用于纳米孔道分析的四通道电化学传感器设计与仿真

纳米孔道技术是一种基于空间限域的超灵敏的单分子分析技术.通过研究单个分子限域于纳米孔道中所产生的离子电流的变化,可在单分子尺度上获取其结构、尺寸、电性及与孔道间弱相互作用的信息.目前主要应用的纳米孔道测量仪器单次实验仅能测量单个纳米孔道,其检测通量较低.本文基于实验室前期自主设计研制的单通道纳米孔道测量仪器Cube-D2上,比较研究了两种互阻放大器的测量特性,从而选择了合适的测量电路设计了四通道电化学传感器放大电路.进一步通过仿真验证了四通道电化学传感器设计方案的可行性,为阵列化高通量纳米孔道单分子电化学测量仪器的设计提供了理论基础.

Design and simulation of four-channel electrochemical sensors for nanopore analysis

Nanopores utilize their confined space for achieving single-molecule detection.Reading the ionic fluctuation induced by a single molecule inside nanopores reveals characteristics of individual molecules such as structures,sizes,electrical properties and interactions.Most of the current commercialized instrumentations for nanopores contains only one recording channel,which acquire the ionic blockages only from a single nanopore in the single experiment.Therefore,the low throughput limits the application of nanopore detection in the real application.In this study,we designed and discussed the appropriate circuit configuration for multi-channel nanopore measurements.Two kinds of trans-impedance amplifiers were compared aiming to improve the throughput of the home-made single-channel electrochemical sensor instrument Cube-D2.Moreover,the bandwidth,noise and bias voltage supply of these circuits are explored and optimized by using circuit simulation softwares,which verifies the feasibility of our design in multichannel nanopore instrumentations.