A solid phase radioimmunoassay for triiodothyronine using antibody-immobilized serum albumin microspheres

A solid phase radioimmunoassay for triiodothyronine (T₃) has been developed using antibody-immobilized serum albumin microspheres. Antibody albumin microspheres were prepared using a spinning disc aerosol generator. The low density of the antibody-albumin microspheres gives greater mobility for the particles there by ensuring better kinetics to the antigen-antibody reaction. The assay has a single incubation of one hour at 37° C and the separation of the antigen-antibody complex is accomplished by centrifugation. The sensitivity of this assay is 0.3 ng/cm³ and has a range of 0.3–4 ng/cm³.