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Preparation and some properties of porcine chymotrypsinogen
Authors:M. T. Proskuryakov
Affiliation:(1) Institute of the Chemistry of Natural Compounds, Kuba Medical Institute, AS USSR
Abstract:Summary A homogeneous preparation of chymotrypsinogen has been obtained by the fractional salting out of an acid extract of porcine pancreas and subsequent chromatography on a column of CM-cellulose. The isoelectric point of the enzyme has been found to be pH 8.8 and the optimum value of the pH for activation with trypsin 7.6. The enzyme is most stable in solutions with pH 3.0.
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