| 吐温-20敏化甲基蓝-蛋白质散射光谱研究与应用 |
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| 引用本文： | 敖登高娃,阿娟,陶玉龙. 吐温-20敏化甲基蓝-蛋白质散射光谱研究与应用[J]. 分析科学学报, 2009, 25(4) |
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| 作者姓名： | 敖登高娃 阿娟 陶玉龙 |
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| 作者单位： | 1. 内蒙古大学化学化工学院,呼和浩特,010021
2. 内蒙古农业大学理学院,呼和浩特,010018 |
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| 摘 要： | 在非离子表面活性剂吐温-20存在下,甲基蓝与蛋白质作用形成离子缔合物,使共振瑞利散射(RRS)急剧增强.研究了相应的光谱特征、影响因素和适宜的反应条件.在pH=4.0和离子强度0.5 mmol5L-1的条件下, 甲基蓝与蛋白质作用在347 nm波长处产生特征RRS,吐温-20进一步敏化该RRS.不同蛋白质在一定浓度范围内与吐温-20敏化的散射强度呈线性关系,检出限在12.3～30.1 μg5L-1范围内.方法用于合成样品以及实际生物样品中蛋白质含量的测定, 结果满意.
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| 关 键 词： | 甲基蓝 蛋白质 吐温-20 共振瑞利散射 |
Spectrophotometric Study of Methyl Blue-protein Resonant System Enhanced by Tween-20 and Its Application |
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| AODENG Gao-wa,A Juan,TAO Yu-long. Spectrophotometric Study of Methyl Blue-protein Resonant System Enhanced by Tween-20 and Its Application[J]. Journal of Analytical Science, 2009, 25(4) |
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| Authors: | AODENG Gao-wa A Juan TAO Yu-long |
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| Affiliation: | AODENG Gaowa1,A Juan2,TAO Yu-long1(1.College of Chemistry and Chemical Engineering,Inner Mongolia University,Hohhot 010021,2.College of Science,Inner Mongolia Agriculture University,Hohhot 010018) |
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| Abstract: | In the presence of non-ionic surfactant Tween-20,methyl blue(MB) could react with protein and form ion-association complex,leading to great enhancement of resonance rayleigh scattering(RRS) intensity.The corresponding characteristics of the spectra,the influencing factors and the optimum conditions of the reaction were studied.Under the conditions of pH=4.0 and ionic strength of 0.5 mmol5L-1,the interaction of MB with protein resulted in enhanced RRS signal at 347 nm.It was found that the interaction was s... |
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| Keywords: | Methyl blue Protein Tween-20 Resonance rayleigh scattering |
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