Homonuclear two-dimensional 1H NMR of proteins. Experimental procedures |
| |
| Affiliation: | 1. Department of Orthopaedic Surgery, Orthopaedic Research & Education Center (OREC), School of Medicine, 1 Rimini Street, Attikon University Hospital, Haidari, 124 62, Athens, Greece;2. Department of Orthopaedic Surgery, School of Medicine, European University Cyprus, Nicosia, Cyprus |
| |
| Abstract: | Experimental techniques used for homonuclear 2D 1H NMR studies of proteins are described. A brief survey of the general strategy for structural studies of proteins by 2D NMR is included. The main part of the paper discusses guidelines for the selection of experimental techniques, the elimination of artifacts and unwanted peaks in protein 2D 1H NMR spectra, suppression of the solvent line in H2O solutions, experimental parameters, numerical data processing before and after Fourier transformation, and suitable presentations of complex 2D NMR spectra. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
