| 小麦液泡膜H~ -ATPase的Ca~(2 )激活特性 |
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| 引用本文： | 王志强,赵芳,董彩华,王延枝. 小麦液泡膜H~ -ATPase的Ca~(2 )激活特性[J]. 武汉大学学报(理学版), 1999, 0(6) |
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| 作者姓名： | 王志强 赵芳 董彩华 王延枝 |
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| 作者单位： | 武汉大学生命科学学院!武汉430072 |
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| 基金项目： | 国家自然科学基金!资助项目(39570434) |
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| 摘 要： | 纯化小麦液泡膜H -ATPase,测定了纯化的小麦液泡膜H -ATPase 受Mg2 和Ca2 双重激活的特性及两种激活状态下酶的特性. Mg2 激活时酶水解活性受DCCD的抑制,受磷脂酰丝氨酸(PS)影响明显,ATP水解和泵质子活性相偶联.而在Ca2 激活时酶水解活性不受DCCD抑制,受PS影响微弱,Ca2 激活时ATP水解与泵质子活性解偶联. Mg2 和Ca2 对小麦液泡膜H -ATPase的激活作用不具有叠加效应.
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| 关 键 词： | Ca~(2 )激活 液泡膜H~ -ATPase 小麦 |
Characterization of Ca~(2 ) Stimulation of Wheat Tonoplast H~ -ATPase |
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| WANG Zhi|qiang,ZHAO Fang,DONG Cai|hua,WANG Yan|zhi. Characterization of Ca~(2 ) Stimulation of Wheat Tonoplast H~ -ATPase[J]. JOurnal of Wuhan University:Natural Science Edition, 1999, 0(6) |
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| Authors: | WANG Zhi|qiang ZHAO Fang DONG Cai|hua WANG Yan|zhi |
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| Abstract: | The stimulations of wheat tonoplast H |ATPase by both Mg 2 and Ca 2 were measured on the base of purification of the enzyme. The characterizations of the Mg 2 |stimulated and Ca 2 |stimulated H |ATPase were studied. Mg 2 |stimulated H |ATPase is inhibited by DCCD, and is greatly affected by PS. ATP hydrolysis and the H pumping of the Mg 2 |stimulated H |ATPase are coupled. But the Ca 2 |stimulated H |ATPase is insensitive to DCCD, and is only slightly affected by PS. ATP hydrolysis and the H pumping of the Ca 2 |stimulated H |ATPase are uncoupled. The Mg 2 stimulation and Ca 2 stimulation of the wheat tonoplast H |ATPase have effects on each other. |
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| Keywords: | Ca 2 stimultion tonoplast H |ATPase wheat |
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