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Studies on substrates for the spectrophotometric determination of hydrogen peroxide based on the catalytic effects of peroxidase-like metalloporphyrins
Authors:Fang Wang   Yan-Zhong Wu   Xiao-Wei Wu   Shu-Seng Shong  Yun-Xiang Ci
Affiliation:(1) Beijing Center of Physical and Chemical Analysis, 27 North Road, Xi San Huan, 100081 Beijing, People's Republic of China;(2) Library, Beijing Institute of Technology, 100081 Beijing, People's Republic of China;(3) Department of Chemistry, Peking University, 100871 Beijing, People's Republic of China
Abstract:Summary The effects have been studied of ten phenol derivatives as chromogenic substrates for the hydrogen peroxide oxidation of 4-aminoantipyrine, catalysed by horse radish peroxidase or peroxidase-like metalloporphyrins. Sodium 2-hydroxy-3,5-dichlorobenzene-sulfonate was found to be the most suitable substrate for the determination of H2O2.
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