Nuclease tolerant FRET probe based on DNA-quantum dot conjugation. |
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| Authors: | Daisuke Onoshima Noritada Kaji Manabu Tokeshi Yoshinobu Baba |
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| Institution: | Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan. onoshima.daisuke@d.mbox.nagoya-u.ac.jp |
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| Abstract: | We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI). |
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