| Nafion 膜固定的新亚甲基蓝为介体生物传感器 |
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| 引用本文： | 孙康,王丽平,陶玲,陈孝康.Nafion 膜固定的新亚甲基蓝为介体生物传感器[J].化学研究与应用,2004,16(2):191-194. |
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| 作者姓名： | 孙康 王丽平 陶玲 陈孝康 |
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| 作者单位： | 四川教育学院化学系,四川,成都,610041;四川大学华西医院普外一科,四川,成都,610041 |
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| 摘 要： | 以Nafion膜固定的新亚甲基蓝为辣根过氧化物酶和玻碳电极间的电子传递介体，制成电流型单酶过氧化氢生物传感器和双酶葡萄糖生物传感器。探讨了工作电位、pH值、温度和干扰物质等对生物传感器的影响。
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| 关 键 词： | 生物传感器 Nafion 新亚甲基蓝 过氧化氢 葡萄糖 |
| 文章编号： | 1004-1656(2003)02-0191-04 |
| 修稿时间： | 2002年4月9日 |
Biosensors for hydrogen peroxide and glucose based on new methylene blue incorporated into nafion film as mediator |
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| SUN Kang,WANG Li-ping,TAO Ling,CHEN Xiao-kang.Biosensors for hydrogen peroxide and glucose based on new methylene blue incorporated into nafion film as mediator[J].Chemical Research and Application,2004,16(2):191-194. |
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| Authors: | SUN Kang WANG Li-ping TAO Ling CHEN Xiao-kang |
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| Institution: | SUN Kang~1,WANG Li-ping~2,TAO Ling~1,CHEN Xiao-kang~1 |
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| Abstract: | An amperometric biosensor highly sensitive to H_2O_2 and a bienzymebased biosensor highly sensitive to glucose were constructed,based on new methylene blue incorporated into a Nafion membrane as the electron transfer mediator between horseradish peroxide and glass carbon electrode.Characteristics of the biosensors were evaluated with respect to dependence on applied potential,solution pH,temperature and interferents.The biosensors displayed high selectivity,high sensitivity and fast response. |
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| Keywords: | biosensor Nafion new methylene blue hydrogen peroxide glucose |
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