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DNA ejection from bacteriophage: Towards a general behavior for osmotic-suppression experiments |
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| Authors: | M Castelnovo A Evilevitch |
| Institution: | (1) Laboratoire Joliot-Curie et Laboratoire de Physique, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon cedex 07, France;(2) Department of Biochemistry, Center of Chemistry and Chemical Engineering, Lund University, P.O. Box 124, S-221 00 Lund, Sweden |
| Abstract: | We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and λ using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed. |
| Keywords: | 82 35 Lr Physical properties of polymers 82 39 Pj Nucleic acids DNA and RNA bases 82 39 Rt Reactions in complex biological systems 82 60 Lf Thermodynamics of solutions |
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