| 百合皂苷的提取﹑纯化及其鉴定 |
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| 引用本文： | 吴晓斌,任凤莲,邱昌桂,吴泓毅.百合皂苷的提取﹑纯化及其鉴定[J].广州化学,2005,30(2):36-40. |
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| 作者姓名： | 吴晓斌 任凤莲 邱昌桂 吴泓毅 |
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| 作者单位： | 中南大学,化学化工学院,湖南,长沙 410083 |
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| 摘 要： | 用正交实验法对百合总皂甙的提取工艺中温度、乙醇浓度、提取系统中固液比例、回流时间和提取次数5个因素进行研究,优选出简便可靠、且适合工业化生产的百合总皂甙的提取工艺。其最佳提取工艺条件是:温度为70℃,乙醇浓度为80%,固液比例为1∶6,提取时间3h,提取次数3次。用AB-8大孔吸附树脂分离、乙醚-丙酮分步沉淀得到纯百合皂苷。通过TCL薄层层析检测和红外光谱对其结构进行了初步鉴定。
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| 关 键 词： | 百合 总皂甙 提取 纯化 鉴定 |
| 文章编号： | 1009-220X(2005)02-0036-05 |
| 修稿时间： | 2004年7月28日 |
Extraction, Purification and Characterization of Lilium Brownii |
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| WU Xiao-bing,REN Feng-lian,QIU Chang-gui,WU Hong-Yi.Extraction, Purification and Characterization of Lilium Brownii[J].Guangzhou Chemistry,2005,30(2):36-40. |
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| Authors: | WU Xiao-bing REN Feng-lian QIU Chang-gui WU Hong-Yi |
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| Institution: | WU Xiao-bing,REN Feng-lian*,QIU Chang-gui,WU Hong-yi |
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| Abstract: | According to orthogonal design,five factors in extraction, i.e. ethanol, temperature,solid/liquid ratio of extracting system,reflux times and extraction times, were studied to optimize a process for extracting lilium brownii. It is simple,reliable and suitable for industrialized production. The results showed that the optimum extraction condition was that at temperature of 70℃, concentration of alcohol 80%,solid/liquid ratio of 1∶ 6 and reflux time of 3 hours with 3 repetitions. Saponin was separated and purified by AB-8 porous resin absorption and step-sedimentation with acetone-ether. Its structure was charaterized by IR spectrum. |
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| Keywords: | lilium brownii saponins extraction purification charaterization |
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