Flow injection spectrophotometric determination of vitamin E in pharmaceuticals,milk powder and blood serum using potassium ferricyanide-Fe(Ⅲ) detection system

<正>A simple and sensitive flow injection spectrophotometric method is reported for the determination of vitamin E using potassium ferricyanide-Fe(Ⅲ) detection system.In the presence of vitamin E,Fe(Ⅲ)/ferricyanide reduces.The in situ reduced ions are then reacted with unreduced portion of ferricyanide/Fe(Ⅲ) to make soluble Prussian blue,which is monitored at absorption wavelength of 735 nm.Linear calibration graph was obtained in the concentration range of 0.1-40μg mL~(-1).The relative standard deviations (n=4) were in the range of 1.1-3.6%,with limits of detection(3 s blank) of 0.04μg mL~(-1).The proposed method allowed 12 injections h~(-1).The method is applied to determine vitamin E in pharmaceuticals,infant milk and blood serum samples using hexane extraction with the recoveries in the range of 93±3 to 97.5±4%.The method is validated using certified reference materials SRM 968c for blood serum samples.

Flow injection spectrophotometric determination of vitamin E in pharmaceuticals, milk powder & blood serum using potassium ferricyanide-Fe(III) detection system

A simple and sensitive flow injection spectrophotometric method is reported for the determination of vitamin E using potassium ferricyanide-Fe(III) detection system. In the presence of vitamin E, Fe(III)/ferricyanide reduces. The insitu reduced ions are then reacted with unreduced portion of ferricyanide/Fe(III) to make soluble Prussian blue, which is monitored at absorption wavelength of 735 nm. Linear calibration graph was obtained in the concentration range of 0.1–40 µg mL-1. The relative standard deviations (n = 4) were in the range of 1.1 – 3.6%, with limits of detection (3s blank) of 0.04 µg mL-1. The proposed method allowed 12 injections h-1. The method is applied to determine vitamin E in pharmaceuticals, infant milk and blood serum samples using hexane extraction with the recoveries in the range of 93±3 to 97.5±4%. The method is validated using certified reference materials SRM 968c for blood serum samples.