Spatial temperature gradient capillary electrophoresis for DNA mutation detection. |
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| Authors: | L Zhu H K Lee B Lin E S Yeung |
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| Affiliation: | Department of Chemistry National University of Singapore, Republic of Singapore. |
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| Abstract: | A continuous spatial temperature gradient was established in capillary electrophoresis by using a simple temperature control device. The temperature profile along the capillary was predicted by theoretical calculations. A nearly linear spatial temperature gradient was established and applied to DNA mutation detection. By spanning a wide temperature range, it was possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures. |
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