| 用Dot-ELISA检测马鼻肺炎病毒抗体的研究 |
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| 引用本文： | 晁群芳. 用Dot-ELISA检测马鼻肺炎病毒抗体的研究[J]. 新疆大学学报(理工版), 2004, 21(3): 292-294 |
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| 作者姓名： | 晁群芳 |
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| 作者单位： | 新疆大学生命科学与技术学院，新疆乌鲁木齐830046 |
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| 摘 要： | 用从德国引进的马鼻肺炎(EHV-1)标准毒株经BHK-21细胞传代增殖，免疫家兔，制备高免血清，提取IgG并用辣根过氧化物酶(HRP)进行标记，建立了能够大量、快速、特异地检测马鼻肺炎的斑点-酶联免疫吸附实验(Dot-ELISA)方法，并应用此方法对100份马血、2份野马流产胎儿病料及从野马流产胎儿病料中分离的毒株PH93-1进行了检测，结果证明，本方法具有较好的特异性和敏感性，而且操作简便、快速，是一种适用于马鼻肺炎的诊断、流行病学调查、检疫和监测的良好方法。
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| 关 键 词： | 马鼻肺炎病毒 Dot-ELISA 检测 |
| 文章编号： | 1000-2839(2004)03-0292-03 |
| 修稿时间： | 2003-01-06 |
Detection of the Serum Anti-body Against Equine Rhinopneumnoitis Virus by Dot-ELISA |
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| CHAO Qun-fang. Detection of the Serum Anti-body Against Equine Rhinopneumnoitis Virus by Dot-ELISA[J]. Journal of Xinjiang University(Science & Engineering), 2004, 21(3): 292-294 |
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| Authors: | CHAO Qun-fang |
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| Abstract: | A sensitive and specific diagnostic method for detecting equine rhionpneumonitis virus is developed using dot-ELISA . Equine rhinopneumonitis virus is propagated on BHK-21 cell successfully. The hyperimmune rabbit anti-equine rhinopneumonitis virus IgG raised against equine rhinopneumonitis virus antigen is used as capture anti-body and purified rabbit anti-equine rhinopneumonitis virus IgG labeled with horseradish peroxidase (HRP). The result in the detection of 100 sera of horse shows that they are all negative and sensitive. The virus, (PH93-1) which has been isolated from Prezwalskii horse and two abortive fetuses is examined. |
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| Keywords: | equine rhinopneumnoitis virus Dot-ELISA detetion |
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